Nuclear import in permeabilized protoplasts from higher plants has unique features.
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چکیده
منابع مشابه
Nuclear import in permeabilized protoplasts from higher plants has unique features.
The import of proteins into the nucleus is a poorly understood process that is thought to require soluble cytosolic factors in vertebrates and yeast. To test this model in plants and to identify components of the import apparatus, we developed a direct in vitro nuclear import assay by using tobacco protoplasts that were permeabilized without detergents such as digitonin or Triton X-100. Substra...
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We have developed an in vitro system involving digitonin-permeabilized vertebrate cells to study biochemical events in the transport of macromolecules across the nuclear envelope. While treatment of cultured cells with digitonin permeabilizes the plasma membranes to macromolecules, the nuclear envelopes remain structurally intact and nuclei retain the ability to transport and accumulate protein...
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ژورنال
عنوان ژورنال: The Plant Cell
سال: 1996
ISSN: 1040-4651,1532-298X
DOI: 10.1105/tpc.8.8.1337